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JoVE Journal Genetics
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Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

Article DOI: 10.3791/59428-v • 08:10 min • May 2nd, 2019
May 2nd, 2019 •
*1, *1,2, 1, 1, 1, 1, 1,3, 1
1Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, 2Jilin Agricultural University, 3Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses
* These authors contributed equally

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Summary
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A pan-lyssavirus nested reverse transcription polymerase chain reaction has been developed to detect specifically all known lyssaviruses. Validation using rabies brain samples of different animal species showed that this method has a sensitivity and specificity equivalent to the gold standard fluorescent antibody test and could be used for routine rabies diagnosis.

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Nested RT-PCR Lyssaviruses Universal Detection Clinical Rabies Diagnosis Surveillance PCR Carry-over Contamination Negative Control Positive Control RNA Extraction Reverse Transcription Rabies Contamination Peptide Chain Ribonuclease Free Materials
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