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Journal / Developmental Biology / iPSC 유래 근육 전조제에서 디스트로핀 발현 복원에 있는 CRISPR/Cas9…
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JoVE Journal Developmental Biology
iPSC 유래 근육 전조제에서 디스트로핀 발현 복원에 있는 CRISPR/Cas9 기술
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iPSC 유래 근육 전조제에서 디스트로핀 발현 복원에 있는 CRISPR/Cas9 기술

Article DOI: 10.3791/59432-v • 07:44 min • September 14th, 2019
September 14th, 2019 •
1, 1, 1, 1, 1
1Medical College of Georgia, Augusta University

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여기서, 우리는Dmdmdx 마우스 유래 피부 섬유아세포로부터 iPSC에서 디스트로핀 발현을 복원하고 Tet-on MyoD 활성화 시스템을 사용하여 iPSC를 myogenic 전구 세포(MPC)로 직접 분화하는 Cas9 기반 exon23 결실 프로토콜을 제시한다.

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발달 생물학 문제 151 유도 만능 줄기 세포 iPSC CRISPR/Cas9 DMD 디스트로핀 근생 선조 분화
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