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JoVE Journal Developmental Biology
CRISPR/Cas9 teknologi i gjenopprette Dystrofingenet uttrykk i iPSC-avledet Muscle forfedre
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CRISPR/Cas9 teknologi i gjenopprette Dystrofingenet uttrykk i iPSC-avledet Muscle forfedre

Article DOI: 10.3791/59432-v • 07:44 min • September 14th, 2019
September 14th, 2019 •
1, 1, 1, 1, 1
1Medical College of Georgia, Augusta University

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Her presenterer vi en Cas9-basert exon23 sletting protokoll for å gjenopprette dystrofingenet uttrykk i iPSC fra DMDMDX mus-avledet hud fibroblaster og direkte differensiere iPSCs til myogenic stamceller (MPC) ved hjelp av tet-on MyoD aktiveringssystem.

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Utviklingsbiologi indusert Pluripotent stamceller iPSC CRISPR/Cas9 DMD dystrofingenet myogenic stamfar differensiering
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