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Ikke-invasiv strategier for kronisk manipulering av DREADD-kontrollerte neuronal aktivitet

Article DOI: 10.3791/59439-v • 08:28 min • August 25th, 2019
August 25th, 2019 •
1, 1, 2, 1, 1
1Section on Light and Circadian Rhythms (SLCR), National Institute of Mental Health, 2Howard Hughes Medical Institute, Department of Neuroscience, Scripps Research Institute

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Her beskriver vi to ikke-invasive metoder for å kronisk kontrollere neuronal aktivitet ved hjelp av chemogenetics i mus. Eye-DROPS ble brukt til å levere Clozapine-N-oksid (CNO) daglig. Vi beskriver også to metoder for langvarig administrering av CNO i drikkevann. Disse strategiene for kronisk neuronal kontroll krever minimalt inngrep redusere dyrs stress.

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Nevrovitenskap ikke-invasiv metoder kronisk CNO chemogenetic DREADDs ekstern neuronal kontroll øye-dråper drikkevann mus
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