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Microscopie d'imagerie hyperspectrale à balayage d'excitation pour discriminer efficacement les signaux de fluorescence
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Microscopie d'imagerie hyperspectrale à balayage d'excitation pour discriminer efficacement les signaux de fluorescence

Article DOI: 10.3791/59448-v • 07:34 min • August 22nd, 2019
August 22nd, 2019 •
1,2,3, 2,3, 2,3, 1,2,3
1Department of Chemical and Biomolecular Engineering, University of South Alabama, 2Center for Lung Biology, University of South Alabama, 3Department of Pharmacology, University of South Alabama

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L'imagerie spectrale est devenue une solution fiable pour l'identification et la séparation des signaux de fluorescence multiples dans un seul échantillon et peut facilement distinguer les signaux d'intérêt du fond ou de l'autofluorescence. L'imagerie hyperspectrale à balayage d'excitation améliore cette technique en diminuant le temps d'acquisition d'image nécessaire tout en augmentant simultanément le rapport signal-bruit.

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Ingénierie Numéro 150 hyperspectral spectroscopie excitation fluorescence microscopie autofluorescence imagerie filtre réglable film mince
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