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Excitation-scanning Hyperspektrala Imaging mikroskopi att effektivt diskriminera fluorescens signaler

Article DOI: 10.3791/59448-v • 07:34 min • August 22nd, 2019
August 22nd, 2019 •
1,2,3, 2,3, 2,3, 1,2,3
1Department of Chemical and Biomolecular Engineering, University of South Alabama, 2Center for Lung Biology, University of South Alabama, 3Department of Pharmacology, University of South Alabama

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Spectral Imaging har blivit en pålitlig lösning för identifiering och separation av multipla fluorescenssignaler i ett enda prov och kan lätt urskilja signaler av intresse från bakgrund eller autofluorescence. Excitation-scanning Hyperspektrala Imaging förbättrar denna teknik genom att minska den nödvändiga bilden förvärvs tid samtidigt öka signal-brus-förhållande.

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Teknik Hyperspektrala spektroskopi excitation fluorescens mikroskopi autofluorescence Imaging avstämbart filter thin film
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