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Biochemistry

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JoVE Journal Biochemistry
Verbesserung der Transplantation von humaninduzierten pluripotenten Stammzellen abgeleiteten Kardiomyozyten über eine transiente Hemmung der Rho Kinase Aktivität
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Verbesserung der Transplantation von humaninduzierten pluripotenten Stammzellen abgeleiteten Kardiomyozyten über eine transiente Hemmung der Rho Kinase Aktivität

Article DOI: 10.3791/59452-v • 08:00 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1,2, 1, 1,3, 1, 1, 1, 2, 1, 1
1Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham, 2Division of Cardiovascular Diseases, School of Medicine, School of Engineering, University of Alabama at Birmingham, 3Department of Cardiac Surgery, The Second Xiangya Hospital of Central South University

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In diesem Protokoll zeigen wir und erläutern, wie man humaninduzierte pluripotente Stammzellen zur Kardiomyozytendifferenzierung und -reinigung verwendet und weiter, wie die Transplantationseffizienz mit dem Rho-assoziierten Proteinkinase-Inhibitor verbessert werden kann. Vorbehandlung in einem Maus-Myokardinfarktmodell.

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Biochemie Ausgabe 149 pluripotente Stammzellen Kardiomyozyten Zelltherapie Myokardinfarkt Rhokinase ROCK-Hemmer
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