JoVE Journal
Immunology and Infection
Immunology and Infection
This content is Open Access.
Chapters
Summary
In this article, we present two complementary methods to measure specific rates of translational error and mistranslation in the model mycobacterium, Mycobacterium smegmatis, using gain-of-function reporter systems. The methods can be employed to measure accurate error rates in low throughput or relative error rates in a more high-throughput setting.