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Journal / Genetics / 多点PCR和毛细血管电泳对鱼类致病菌的多点变量数串联重复分析…
JoVE Journal
Genetics

This content is Open Access.

JoVE Journal Genetics
多点PCR和毛细血管电泳对鱼类致病菌的多点变量数串联重复分析
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多点PCR和毛细血管电泳对鱼类致病菌的多点变量数串联重复分析

Article DOI: 10.3791/59455-v • 10:33 min • June 17th, 2019
June 17th, 2019 •
1, 1, 1,2
1Fish Health Research Group, Norwegian Veterinary Institute, 2University of Bergen

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这里介绍的多位数可变数串联重复分析(MLVA)测定使鱼类致病菌Yersinia ruckeri的廉价、坚固和便携式高分辨率基因分型成为可能。从纯培养基开始,该测定采用多路PCR和毛细管电泳,为下游应用生产十位MLVA型材。

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遗传学 问题 148,耶尔西尼亚鲁克里 耶尔西尼病 MLVA 基因分型 分子流行病学 细菌性鱼类病原体 大西洋鲑鱼 虹鳟鱼
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