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JoVE Journal Bioengineering
Una strategia convergente per la generazione di una libreria cDNA virtualmente in sequenza da ostriche del Pacifico senza riferimenti
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Una strategia convergente per la generazione di una libreria cDNA virtualmente in sequenza da ostriche del Pacifico senza riferimenti

Article DOI: 10.3791/59462-v • 12:14 min • June 13th, 2019
June 13th, 2019 •
1, 1, 1, 1, 1, 1, 2, 1
1Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, 2College of Life Science, Nanjing Agricultural University

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Descriviamo una strategia su come usare campioni di RNA da campioni di ostriche del Pacifico senza riferimenti e valutiamo il materiale genetico confrontandosi con i dati del genoma pubblicamente disponibili per generare una libreria cDNA virtualmente sequenziata.

Tags

Bioingegneria Problema 148 Ostriche del Pacifico Crassostrea gigas Magallana gigas libreria cDNA biosintesi UDP-xylose glicobiologia dei molluschi
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