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JoVE Journal Cancer Research
Snelle aanmaak van primaire Murine melanocyten en fibroblast-culturen
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Snelle aanmaak van primaire Murine melanocyten en fibroblast-culturen

Article DOI: 10.3791/59468-v • 06:32 min • June 26th, 2019
June 26th, 2019 •
1, 1, 1,2
1Department of Cancer Biology and Genetics, The Ohio State University, 2Department of Molecular Genetics, The Ohio State University

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Dit protocol schetst een snelle methode voor het gelijktijdig genereren van melanocyt en fibroblast culturen uit de huid van 0-4 dag oude muizen. Deze primaire culturen kunnen in vitro worden gehandhaafd en gemanipuleerd om een verscheidenheid aan fysiologisch relevante processen te bestuderen, waaronder huidcelbiologie, pigmentatie, wondgenezing en melanoom.

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Kankeronderzoek probleem 148 melanocyte muis huid isolatie celkweek
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