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Génération rapide de cultures de murine primaire et de fibroblaste

Article DOI: 10.3791/59468-v • 06:32 min • June 26th, 2019
June 26th, 2019 •
1, 1, 1,2
1Department of Cancer Biology and Genetics, The Ohio State University, 2Department of Molecular Genetics, The Ohio State University

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Ce protocole décrit une méthode rapide pour générer simultanément des cultures de mlanocytes et de fibroblastes à partir de la peau de souris de 0 à 4 jours. Ces cultures primaires peuvent être maintenues et manipulées in vitro pour étudier une variété de processus physiologiquement pertinents, y compris la biologie des cellules de la peau, la pigmentation, la cicatrisation des plaies et le mélanome.

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Recherche sur le cancer numéro 148 mlanocyte souris peau isolement culture cellulaire
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