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Journal / Bioengineering / 快速生长的纳氏弧菌的无细胞蛋白表达…
JoVE Journal
Bioengineering

This content is Open Access.

JoVE Journal Bioengineering
快速生长的纳氏弧菌的无细胞蛋白表达
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快速生长的纳氏弧菌的无细胞蛋白表达

Article DOI: 10.3791/59495-v • 08:52 min • March 14th, 2019
March 14th, 2019 •
1,2, 1, *1, *1,2
1Department of Genetics, Harvard Medical School, 2Wyss Institute for Biologically Inspired Engineering
* These authors contributed equally

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Summary
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无细胞表达系统是用于高吞吐量合成和筛选重要蛋白质的功能强大且经济高效的工具。在这里, 我们描述了使用纳利根弧菌快速生产的无细胞蛋白表达系统的制备, 使用质粒 dna, 线性 dna 和 mrna 模板。

Tags

生物工程 第145期 纳布里根弧菌 粗细胞提取物 无细胞系统 tx-tl 体外蛋白质合成 高通量筛选
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