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Kunstmatige RNA polymerase II-rek complexen voor het ontleden van co-transcriptionele RNA-verwerkings gebeurtenissen
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Kunstmatige RNA polymerase II-rek complexen voor het ontleden van co-transcriptionele RNA-verwerkings gebeurtenissen

Article DOI: 10.3791/59497-v • 10:59 min • May 13th, 2019
May 13th, 2019 •
1, 1,2, 1,2
1Stowers Institute for Medical Research, 2Department of Biochemistry and Molecular Biology, Kansas University Medical Center

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Hier beschrijven we de assemblage van RNA polymerase II (Pol II) rek complexen waarvoor alleen korte synthetische DNA en RNA oligonucleotiden en gezuiverde Pol II. Deze complexen zijn nuttig voor het bestuderen van mechanismen die onderliggende co-transcriptionele verwerking van transcripties in verband met het Pol II rek complex.

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Genetica probleem 147 mRNA synthese RNA polymerase II transcriptie verlenging capping enzym CTD kinase synthetische transcriptie bubbels kunstmatige rek complexen TFIIH co-transcriptionele RNA-verwerking
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