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Kunstig RNA polymerase II forlengelse komplekser for dissekere co-transcriptional RNA prosessering hendelser

Article DOI: 10.3791/59497-v • 10:59 min • May 13th, 2019
May 13th, 2019 •
1, 1,2, 1,2
1Stowers Institute for Medical Research, 2Department of Biochemistry and Molecular Biology, Kansas University Medical Center

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Her beskriver vi monteringen av RNA polymerase II (Pol II) forlengelse komplekser som kun krever kort syntetisk DNA og RNA-oligonukleotider og renset Pol II. Disse komplekser er nyttige for å studere mekanismer underliggende co-transcriptional behandling av transkripsjoner knyttet til Pol II forlengelse kompleks.

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Genetikk mRNA syntese RNA polymerase II transkripsjon forlengelse capping enzym CTD kinase syntetisk transkripsjon bobler kunstige forlengelse komplekser TFIIH co-transcriptional RNA prosessering
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