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Immunology and Infection

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JoVE Journal Immunology and Infection
En in vitro-batch-kultur model til at estimere virkningerne af interventionelle regimer på human fecal microbiota
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En in vitro-batch-kultur model til at estimere virkningerne af interventionelle regimer på human fecal microbiota

Article DOI: 10.3791/59524-v • 07:15 min • July 31st, 2019
July 31st, 2019 •
*1,2,3, *1,2, *1,2, 1,2, 3,4, 5, 1,2
1Department of Internal Medicine- Molecular Medicine, Wake Forest School of Medicine, 2Department of Microbiology and Immunology, Wake Forest School of Medicine, 3Department of Food Science and Technology, Isfahan University of Technology, 4Research Institute for Biotechnology and Bioengineering, College of Agriculture, Isfahan University of Technology, 5Department of Geriatrics and Gerontology, Wake Forest School of Medicine
* These authors contributed equally

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Denne protokol beskriver en in vitro batch-kultur fermenterings system af humant fækal mikrobiota, ved hjælp af inulin (en velkendt prebiotiske og en af de mest udbredte studerede mikrobiota modulatorer) at demonstrere brugen af dette system til at anslå virkningerne af specifikke interventioner på fækal mikrobiota sammensætning og metaboliske aktiviteter.

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Immunologi og infektion anaerob batch kultur kolon gæring in vitro microbiota mikroflora kortkædede fedtsyrer pH
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