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Journal / Immunology and Infection / Skreddersy in vivo cytotoksisitet analyser for å…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Skreddersy in vivo cytotoksisitet analyser for å studere Immunodominance i tumor spesifikke CD8+ T celle svar
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Skreddersy in vivo cytotoksisitet analyser for å studere Immunodominance i tumor spesifikke CD8+ T celle svar

Article DOI: 10.3791/59531-v • 10:13 min • May 6th, 2019
May 6th, 2019 •
1, 1, 1,2,3,4
1Department of Microbiology and Immunology, Western University, 2Department of Medicine, Division of Clinical Immunology and Allergy, Western University, 3Department of Surgery, Division of General Surgery, Western University, 4Lawson Health Research Institute

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Vi beskriver her en flyt flowcytometri-basert in vivo drap analysen som muliggjør undersøkelse av immunodominance i cytotoksisk T-lymfocytter (CTL) svar på en modell svulst antigen. Vi gir eksempler på hvordan denne elegante analysen kan være ansatt for mekanistisk studier og for narkotika effektivitet testing.

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Immunologi og infeksjon CD8+ T celler cytotoksisitet kreft immunodominance tumor-avledet peptider store T antigen regulatoriske T-celler PD-1 Checkpoint hemmere narkotika effektivitet testing Flow flowcytometri
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