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Journal / Developmental Biology / Microscopia de folha de luz isotrópica e análise…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Microscopia de folha de luz isotrópica e análise de linhagem de células automatizadas para catalogar Caenorhabditis elegans Embryogenesis com resolução subcelular
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Microscopia de folha de luz isotrópica e análise de linhagem de células automatizadas para catalogar Caenorhabditis elegans Embryogenesis com resolução subcelular

Article DOI: 10.3791/59533-v • 08:16 min • June 6th, 2019
June 6th, 2019 •
1,5, 1,5, 1,5, 1,5, 1,5, 4,5, 4,5, 4,5, 2,5, 2,5, 4,5, 3,5, 1,5,6
1Department of Neuroscience and Department of Cell Biology, Yale University School of Medicine, 2Developmental Biology Program, Sloan Kettering Institute, 3Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling, University of Connecticut Health Center, 4Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 5WormGUIDES.org, 6Instituto de Neurobiología, Recinto de Ciencias Médicas, Universidad de Puerto Rico

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Aqui, nós apresentamos uma aproximação combinatória usando a microscopia de alta resolução, as ferramentas computacionais, e a rotulagem da único-pilha em embriões vivos de C. elegans para compreender a única dinâmica da pilha durante o neurodevelopment.

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Biologia do desenvolvimento edição 148 diSPIM CytoSHOW deconvolution SpimFusion StarryNite visualização linhagem neurodevelopment C. elegans embriões
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