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Journal / Immunology and Infection / Redning av rekombinant Zika virus fra en…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Redning av rekombinant Zika virus fra en bakteriell kunstig kromosom cDNA Clone
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Redning av rekombinant Zika virus fra en bakteriell kunstig kromosom cDNA Clone

Article DOI: 10.3791/59537-v • 08:10 min • June 24th, 2019
June 24th, 2019 •
1, 1, 1, 2, 1
1Department of Microbiology and Immunology, University of Rochester Medical Center, 2Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid

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Den nylige epidemien av Zika virus fremhever viktigheten av å etablere omvendt genetiske tilnærminger til å utvikle vaksiner og/eller terapeutiske strategier. Her beskriver vi protokollen for å redde en smittsom rekombinant Zika virus fra en full-lengde cDNA klone samlet i en bakteriell kunstig kromosom under kontroll av den menneskelige Cytomegalovirus umiddelbar-tidlig promoter.

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Immunologi og infeksjon Arbovirus Flavivirus Zika virus omvendt genetikk full lengde cDNA smittsomme kloner bakteriell kunstig kromosom cDNA transfeksjoner virus redning
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