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Journal / Immunology and Infection / Rescate del virus del Zika recombinante de un…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Rescate del virus del Zika recombinante de un clon de ADNc cromosómico artificial bacteriano
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Rescate del virus del Zika recombinante de un clon de ADNc cromosómico artificial bacteriano

Article DOI: 10.3791/59537-v • 08:10 min • June 24th, 2019
June 24th, 2019 •
1, 1, 1, 2, 1
1Department of Microbiology and Immunology, University of Rochester Medical Center, 2Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid

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La reciente epidemia del virus del Zika pone de relieve la importancia de establecer enfoques genéticos inversos para desarrollar vacunas y/o estrategias terapéuticas. Aquí, describimos el protocolo para rescatar un virus del Zika recombinante infeccioso de un clon de ADNc de longitud completa ensamblado en un cromosoma artificial bacteriano bajo el control del promotor inmediato-temprano del citomegalovirus humano.

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Inmunología e infección número 148 arbovirus flavivirus virus del Zika genética inversa clones infecciosos de ADNc de longitud completa cromosoma artificial bacteriano transfección de ADNc rescate de virus
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