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Journal / Developmental Biology / Generasjon av Organoids fra mus Extrahepatic…
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JoVE Journal Developmental Biology
Generasjon av Organoids fra mus Extrahepatic galle kanaler
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Generasjon av Organoids fra mus Extrahepatic galle kanaler

Article DOI: 10.3791/59544-v • 09:13 min • April 23rd, 2019
April 23rd, 2019 •
1, 1, 1,2, 1,2, 1
1Departments of Internal Medicine, The University of Michigan, 2Molecular & Integrative Physiology, The University of Michigan

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Denne protokollen beskriver produksjon av en mus extrahepatic galle duct 3-dimensjonale organoid system. Disse biliær organoids kan vedlikeholdes i kultur å studere cholangiocyte biologi. Biliær organoids express markører for både stamfar og biliær celler og består av polarisert epitelceller.

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Utviklingspsykologi biologi problemet 146 biliær progenitor celler cholangiocytes organoids 3-dimensjonale kultur-systemet narkotikarelaterte screening cholangiopathies
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