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Immunology and Infection

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JoVE Journal Immunology and Infection
Kvantificering af prolifererende humane antigen-specifikke CD4+ T-celler ved hjælp af Carboxyfluorescein Succinimidyl ester
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Kvantificering af prolifererende humane antigen-specifikke CD4+ T-celler ved hjælp af Carboxyfluorescein Succinimidyl ester

Article DOI: 10.3791/59545-v • 07:00 min • June 4th, 2019
June 4th, 2019 •
1, 1, 1, 1,2
1Immunology and Diabetes Unit, St. Vincent's Institute of Medical Research, 2Department of Medicine, University of Melbourne, St. Vincent's Hospital

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Præsenteret her er en protokol til måling af prolifererende CD4+ T- celler som reaktion på antigene proteiner eller peptider ved hjælp af Dye fortynding. Denne analyse er særligt følsom over for sjældne antigen-specifikke T-celler og kan modificeres for at lette kloning af antigen-specifikke celler.

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Immunologi og infektion udstedelse 148 human CD4 proliferation CFSE følsom Auto antigen kloning PBMC
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