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JoVE Journal Bioengineering
Fremstilling af Neutrally-ladede, pH-Responsive polymere nanopartikler til Cytosolic siRNA-levering
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Fremstilling af Neutrally-ladede, pH-Responsive polymere nanopartikler til Cytosolic siRNA-levering

Article DOI: 10.3791/59549-v • 09:09 min • May 2nd, 2019
May 2nd, 2019 •
1, 1, 1,2,3
1Department of Chemical Engineering, University of Mississippi, 2Department of BioMolecular Sciences, University of Mississippi, 3Biomedical Engineering Program, University of Mississippi

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Metoder til at forberede og karakterisere de fysisk-kemiske egenskaber og Bioaktivitet af neutrally-ladede, pH-Responsive siRNA nanopartikler præsenteres. Kriterier for vellykkede siRNA nanomediciner såsom størrelse, morfologi, overflade ladning, siRNA lastning, og genhæmning diskuteres.

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Bioengineering RNA-interferens Endosomal flugt Endosomolyse pH-responsiv nanoteknologi polymert nanopartikler Biomedicinsk teknik lægemiddel levering Cancer
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