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JoVE Journal Cancer Research
Dråbe digital TRAP (ddTRAP)
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Dråbe digital TRAP (ddTRAP): tilpasning af telomere REPEAT forstærknings protokol til droplet digital polymerase kædereaktion

Article DOI: 10.3791/59550-v • 06:38 min • May 3rd, 2019
May 3rd, 2019 •
1, 1, 1
1School of Kinesiology, University of Michigan

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Vi har med succes konverteret standard telomere gentagelse forstærknings protokol (TRAP) assay, der skal anvendes i dråbe digitale polymerase kædereaktioner. Denne nye analyse, kaldet ddTRAP, er mere følsom og kvantitativ, giver mulighed for bedre påvisning og statistisk analyse af telomerase aktivitet inden for forskellige humane celler.

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Kræftforskning telomerase TRAP assay dråbe digital PCR kræft enzymatisk aktivitet afsløring aldring kvantitativ biologi
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