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Journal / Cancer Research / Fuldt humant tumor-baserede matrix i…
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Cancer Research

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Fuldt humant tumor-baserede matrix i tredimensionale Spheroid invasion assay
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Fuldt humant tumor-baserede matrix i tredimensionale Spheroid invasion assay

Article DOI: 10.3791/59567-v • 08:15 min • May 7th, 2019
May 7th, 2019 •
1, 1, *1, *1, 1, 1, 1,2,3,4
1Department of Oral and Maxillofacial Diseases, Clinicum, Faculty of Medicine, University of Helsinki, 2Cancer and Translational Medicine Research Unit, University of Oulu, 3Medical Research Center, Oulu University Horspital, 4Helsinki University Hospital
* These authors contributed equally

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Tumor mikromiljø er en væsentlig del af kræft vækst og invasion. For at efterligne karcinomer er der behov for en biologisk relevant menneskelig matrix. Denne protokol introducerer en forbedring for in vitro tredimensionel sfæroide invasion assay ved at anvende en human leiomyoma-baseret matrix. Protokollen introducerer også en computer-baseret celle invasion analyse.

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Kræftforskning tumor mikromiljø billedbehandling kræft Fiji ImageJ ilastik Human myoma-afledte matrix fibrin mus sarkom-afledt matrix kollagen
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