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Journal / Biochemistry / Berigelse af Native lipoprotein partikler med…
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Biochemistry

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JoVE Journal Biochemistry
Berigelse af Native lipoprotein partikler med microRNA og efterfølgende bestemmelse af deres absolutte/relative microRNA indhold og deres cellulære overførselshastighed
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Berigelse af Native lipoprotein partikler med microRNA og efterfølgende bestemmelse af deres absolutte/relative microRNA indhold og deres cellulære overførselshastighed

Article DOI: 10.3791/59573-v • 11:13 min • May 9th, 2019
May 9th, 2019 •
1, 2, 1, 1, 2
1Center for Pathobiochemistry and Genetics, Institute of Medical Chemistry and Pathobiochemistry, Medical University Vienna, 2School of Medical Engineering and Applied Social Sciences, University of Applied Sciences Upper Austria

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Her præsenteres en kvantitativ real-time polymerase kæde reaktions baseret protokol til bestemmelse af det oprindelige Micro-RNA indhold (absolutte/relative) af lipoprotein partikler. Desuden er en metode til at øge mikro-RNA-niveauet, samt en metode til bestemmelse af cellulære optagelse sats af lipoprotein partikler, påvist.

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Biokemi lipoprotein partikler Micro-RNA HDL LDL qPCR absolut indhold CQ værdi overførselshastighed cellulær optagelse
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