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JoVE Journal
Biochemistry

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荧光苏门答腊蛋白对 sumo 修饰蛋白的定位
 
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荧光苏门答腊蛋白对 sumo 修饰蛋白的定位

Article DOI: 10.3791/59576-v 06:23 min April 27th, 2019
April 27th, 2019

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Summary

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SUMO 是一种必需的、高度保守的、类似于泛素的改性剂。在这个协议中, 我们描述了使用一个抗压力重组的 sumo 捕获蛋白 (kmUTAG) 来可视化本地的、未标记的 SUMMO 共轭及其在各种细胞类型中的定位。

Tags

生物化学 第146期 Sumo Smt3 滑-1 sumoylation c . elegans pnt2 萌发酵母 utag sumo-ap进来 ud
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