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Journal / Genetics / Lentiviral medieret genhæmning i humant Pseudoø…
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JoVE Journal Genetics
Lentiviral medieret genhæmning i humant Pseudoø fremstillet i lave fastgørelses plader
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Lentiviral medieret genhæmning i humant Pseudoø fremstillet i lave fastgørelses plader

Article DOI: 10.3791/59578-v • 11:24 min • May 14th, 2019
May 14th, 2019 •
1,2, 1,2, 1,2, 2,3, 2,3, 1,2
1Department of Internal Medicine, Carver College of Medicine, University of Iowa, 2Fraternal Order of Eagles Diabetes Research Center, University of Iowa, 3Roy J. Carver Department of Biomedical Engineering, University of Iowa

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En protokol til oprettelse af genetisk modificerede humane pseudoisletter fra spredte humane ø-celler, der er transduceret af lentivirus med kort hårnål RNA (shRNA), er præsenteret. Denne protokol udnytter let tilgængelige enzym og kultur fartøjer, kan udføres nemt, og producerer genetisk modificerede humane pseudoisletter egnet til funktionelle og morfologiske undersøgelser.

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Genetik shRNA lentivirus insulinsekretion kultur enkelt celle reaggregation
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