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JoVE Journal
Biology

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使用微蒂特萝卜无线电标签进行多维欧大肠杆菌测量 (p) ppGpp,然后是薄层色谱
 
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使用微蒂特萝卜无线电标签进行多维欧大肠杆菌测量 (p) ppGpp,然后是薄层色谱

Article DOI: 10.3791/59595-v 06:30 min June 4th, 2019
June 4th, 2019

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Summary

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微酸度皿中放射性标记细菌培养物的生长有助于高通量采样,从而允许对核苷酸池丰度进行多种技术和生物复制检测,包括 (p) ppGpp。可以监测生理压力来源引起的生长转变以及压力恢复的影响。

Tags

生物学 问题 148 (p) ppGpp 薄层色谱 32P 大肠杆菌 高通量 第二信使
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