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Journal / Biochemistry / Expression und Reinigung von Nuklease-freier…
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Biochemistry

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JoVE Journal Biochemistry
Expression und Reinigung von Nuklease-freier Sauerstofffänger Protocatechuate 3,4-Dioxygenase
Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
Journal (Biochemistry)
Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support
Journal (Biochemistry)
Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support
 
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Expression und Reinigung von Nuklease-freier Sauerstofffänger Protocatechuate 3,4-Dioxygenase

Article DOI: 10.3791/59599-v • 10:14 min • November 8th, 2019
November 8th, 2019 •
1, 1, 1, 1
1Cancer Biology and Genetics, College of Medicine, The Ohio State University

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Protocatechuate 3,4-Dioxygenase (PCD) kann mit seinem Substrat Protocatechuic Säure (PCA) enzymatisch freien diatomischen Sauerstoff aus einem wässrigen System entfernen. Dieses Protokoll beschreibt die Expressions-, Reinigungs- und Aktivitätsanalyse dieses Sauerstoff-Aufräumenzyms.

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Biochemie Ausgabe 153 Chromatographie Protocatechuat-3,4-Dioxygenase (PCD) Nukleasekontamination Protocatechuinsäure (PCA) reaktive Sauerstoffspezies Sauerstoffaufräumsysteme (OSS)
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