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A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity
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A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity

Article DOI: 10.3791/59616-v • 08:04 min • June 11th, 2019
June 11th, 2019 •
*1, *1, 2, 1, 3, 1,2
1Comprehensive Transplant Center, Northwestern University, 2Department of surgery, Feinberg School of Medicine, Northwestern University, 3Weinberg art and science college, Northwestern University
* These authors contributed equally

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Summary
Automatic Translation

This protocol details the surgical steps of a mouse model of vascularized heterotopic spleen transplantation, a technically challenging model that can serve as a powerful tool in studying the fate and longevity of spleen cells, the mechanisms of distinct spleen cell populations in disease progression, and transplant immunity.

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Mouse Model Vascularized Heterotopic Spleen Transplantation Spleen Cell Biology Transplant Immunity Immune Responses Inflammatory Disease Congenic Mice Fate Of Spleen Cells Longevity Of Spleen Cells Function Of Spleen Cells Surgical Platform Anesthetized Donor Mouse Abdominal Area Operating Microscope Sterilize Skin Surgical Tape Abdominal Incision Exposure Of Spleen Cauterize Skin Vessels Expose Spleen Low Temperature Cautery Separate Portal Vein
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