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Journal / Biochemistry / 翻译核糖体亲性纯化(TRAP),用于从体内内皮细胞中分离RNA…
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Biochemistry
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JoVE Journal Biochemistry
翻译核糖体亲性纯化(TRAP),用于从体内内皮细胞中分离RNA
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翻译核糖体亲性纯化(TRAP),用于从体内内皮细胞中分离RNA

Article DOI: 10.3791/59624-v • 08:53 min • May 25th, 2019
May 25th, 2019 •
1,2, 3,4, 1,3, 1, 2, 3, 3,4,5
1Blood Research Institute, Blood Center of Wisconsin, 2Department of Medicine, Medical College of Wisconsin, 3Department of Surgery, The University of Alabama at Birmingham, 4Department of Biomedical Engineering, The University of Alabama at Birmingham, 5GBS Program, Graduate School, The University of Alabama at Birmingham

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我们通过结合RNA纯化结合的增强绿色荧光蛋白(EGFP)的EC特异性遗传标记,直接在小鼠脑、肺和心脏组织中直接从小鼠脑、肺和心脏组织中从血管内皮细胞(EC)中纯化核糖体结合体mRNA的mRNA。.

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生物化学 问题 147 血管生成 动脉分化 血管内皮细胞 翻译核糖体亲性纯化 RNA提取 实时 qPCR
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