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JoVE Journal Biochemistry
Het vertalen van ribosome Affiniteits zuivering (TRAP) voor RNA-isolatie van endotheel cellen in vivo
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Het vertalen van ribosome Affiniteits zuivering (TRAP) voor RNA-isolatie van endotheel cellen in vivo

Article DOI: 10.3791/59624-v • 08:53 min • May 25th, 2019
May 25th, 2019 •
1,2, 3,4, 1,3, 1, 2, 3, 3,4,5
1Blood Research Institute, Blood Center of Wisconsin, 2Department of Medicine, Medical College of Wisconsin, 3Department of Surgery, The University of Alabama at Birmingham, 4Department of Biomedical Engineering, The University of Alabama at Birmingham, 5GBS Program, Graduate School, The University of Alabama at Birmingham

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We presenteren een aanpak om ribosome-gebonden mRNA van vasculaire endotheliale cellen (ECs) direct in muizenhersenen, Long-en hart weefsels te zuiveren via EC-specifieke genetische code van Enhanced Green fluorescentie proteïne (EGFP) in ribosomen in combinatie met RNA-zuivering .

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Biochemie uitgave 147 angiogenese arteriollaire differentiatie vasculaire endotheliale cellen vertalen van ribosoom affiniteits zuivering RNA-extractie real-time qPCR
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