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Journal / Biochemistry / Oversette ribosom affinitet rensing (TRAP) for…
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Biochemistry
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JoVE Journal Biochemistry
Oversette ribosom affinitet rensing (TRAP) for RNA Isolation fra endothelial celler in vivo
Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
Journal (Biochemistry)
Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
Separation of Polymerized Nucleic Acid Constructs by PAGE
Journal (Biochemistry)
Separation of Polymerized Nucleic Acid Constructs by PAGE
 
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Oversette ribosom affinitet rensing (TRAP) for RNA Isolation fra endothelial celler in vivo

Article DOI: 10.3791/59624-v • 08:53 min • May 25th, 2019
May 25th, 2019 •
1,2, 3,4, 1,3, 1, 2, 3, 3,4,5
1Blood Research Institute, Blood Center of Wisconsin, 2Department of Medicine, Medical College of Wisconsin, 3Department of Surgery, The University of Alabama at Birmingham, 4Department of Biomedical Engineering, The University of Alabama at Birmingham, 5GBS Program, Graduate School, The University of Alabama at Birmingham

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Vi presenterer en tilnærming for å rense ribosom-bundet mRNA fra vaskulær endothelial celler (ECs) direkte i mus hjernen, lunge og hjerte vev via EC-spesifikk genetisk kode av forbedret grønt fluorescens protein (EGFP) i ribosomer i kombinasjon med RNA rensing .

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Biokjemi angiogenese arterioler differensiering vaskulær endothelial celler oversette ribosom affinitet rensing RNA utvinning sanntid qPCR
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