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Journal / Cancer Research / 利用生命内成像和固定组织分析评估与癌细胞传播相关的转移门道介导血管渗透性的肿瘤微环境…
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Cancer Research

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JoVE Journal Cancer Research
利用生命内成像和固定组织分析评估与癌细胞传播相关的转移门道介导血管渗透性的肿瘤微环境
An In Vivo Murine Sciatic Nerve Model of Perineural Invasion
Journal (Cancer Research)
An In Vivo Murine Sciatic Nerve Model of Perineural Invasion
High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds
Journal (Cancer Research)
High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds
 
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利用生命内成像和固定组织分析评估与癌细胞传播相关的转移门道介导血管渗透性的肿瘤微环境

Article DOI: 10.3791/59633-v • 09:42 min • June 26th, 2019
June 26th, 2019 •
1,2,3, 1,2,4, 1,2, 1,2, 1,2,4, 1,2,3,4, 1,2,3,5, 1,2,3
1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 2Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, 3Integrated Imaging Program, Albert Einstein College of Medicine, 4Department of Surgery, Montefiore Medical Center, 5Department of Pathology, Montefiore Medical Center

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我们描述了两种方法,用于评估与转移(TMEM)的肿瘤微环境(TMEM)和癌细胞内溢相关的瞬态血管渗透性,使用静脉注射高分子量(155 kDa)dextran小鼠。这些方法包括活体动物的活体成像和使用免疫荧光的固定组织分析。

Tags

回缩,问题148,生命内成像,免疫荧光,血管渗透性,转移肿瘤微环境(TMEM),高分子量dextran,免疫荧光,转移
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