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Immunology and Infection

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JoVE Journal Immunology and Infection

狂犬病ワクチンの効力を評価するインビトロELISA試験

Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

Journal (Immunology and Infection)

Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms

Journal (Immunology and Infection)

Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms

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狂犬病ワクチンの効力を評価するインビトロELISA試験

Article DOI: 10.3791/59641-v • 09:04 min • May 11th, 2020

May 11th, 2020 •

Corinne Jallet¹, Noël Tordo^1,2

¹Unit Antiviral Strategies, OIE Reference Laboratories for Rift Valley Fever Virus & Crimean Congo Hemorrhagic Fever Virus, Institut Pasteur, ²Institut Pasteur de Guinée, Conakry

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ここでは、狂犬病ワクチンにおける免疫原性糖タンパク質の内容を決定するための間接的ELISAサンドイッチ免疫キャプチャについて説明する。この試験は、中和モノクローナル抗体(mAb-D1)を使用して、糖タンパク質三量体を認識します。生産中のワクチン効力の一貫性に従うin vivo NIH試験の代替手段である。

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免疫学と感染症,課題 159 狂犬病ワクチン効力 NIH試験 ELISA法 mAb-D1モノクローナル抗体糖タンパク質の三量体糖タンパク質含有量

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