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JoVE Journal Bioengineering
In vivo duas cores 2-Photon Imaging de células de repórter geneticamente marcadas na pele
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In vivo duas cores 2-Photon Imaging de células de repórter geneticamente marcadas na pele

Article DOI: 10.3791/59647-v • 05:45 min • July 11th, 2019
July 11th, 2019 •
1, 1, 1, 1
1School of Brain and Behavioral Science, Center for Advanced Pain Studies, University of Texas at Dallas

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As alterações morfológicas ocorrem em células de fibroblastos imunes responsivos após a ativação e promovem alterações no recrutamento celular. Utilizando a imagem latente do Photon 2 conjuntamente com uma proteína fibroblástica-específica geneticamente projetada 1 (FSP1)-CRE; tdtomato floxed-parar-floxed (TB/TB) linha do mouse e verde cDNAs Tagged lipopolissacarídeo-FITC, podemos ilustrar a captação altamente específica de lipopolissacaríno em fibroblastos dérmicos e alterações morfológicas in vivo.

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Bioengenharia edição 149 multi-Photon Imaging toll-like receptor 4 genética repórter lipopolissacarde in vivo
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