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Journal / Genetics / Designa automatiserade, hög genom strömning,…
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Genetics

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JoVE Journal Genetics
Designa automatiserade, hög genom strömning, kontinuerlig cell tillväxt experiment med eVOLVER
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Designa automatiserade, hög genom strömning, kontinuerlig cell tillväxt experiment med eVOLVER

Article DOI: 10.3791/59652-v • 07:26 min • May 19th, 2019
May 19th, 2019 •
1,2, 1,2, 1,3, 1,2, 4, 1,2,5
1Biological Design Center, Boston University, 2Department of Biomedical Engineering, Boston University, 3Program in Molecular Biology, Cell Biology and Biochemistry, Boston University, 4Department of Bioengineering, Rice University, 5Wyss Institute for Biologically Inspired Engineering, Harvard University

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Den eVOLVER ramverk möjliggör hög genom strömning kontinuerlig mikrobiell kultur med hög upplösning och dynamisk kontroll över experimentella parametrar. Detta protokoll visar hur man kan tillämpa systemet för att genomföra en komplicerad Fitness experiment, vägleda användare på programmering automatiserad kontroll över många enskilda kulturer, mäta, samla in och interagera med experimentella data i real tid.

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Genetik eVOLVER kontinuerlig kultur mikrobiella populationer Lab Automation tillväxt urval experimentell evolution fitness landskap system bio Logi
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