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Immunology and Infection

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JoVE Journal Immunology and Infection
Robust ligatur-induceret model af murine periodontitis til evaluering af orale neutrofiler
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Robust ligatur-induceret model af murine periodontitis til evaluering af orale neutrofiler

Article DOI: 10.3791/59667-v • 07:15 min • January 21st, 2020
January 21st, 2020 •
1,2, 1,2
1Department of Dental Oncology and Maxillofacial Prosthetics, Princess Margaret Cancer Centre, University Health Network, 2Faculty of Dentistry, University of Toronto

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Denne artikel præsenterer en protokol til etablering af en ligatur-induceret model af murine parodontitis involverer flere maxillær molars, hvilket resulterer i større områder af de involverede gingival væv og knogler til efterfølgende analyse samt reduceret dyre brug. En teknik til at vurdere orale neutrofiler på en måde, der svarer til forsøgspersoner, er også beskrevet.

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Immunologi og infektion periodontitis ligatur gingiva periodontium alveolær knogle dyremodel flow cytometri neutrofile immunologi inflammation
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