Abstract
Special histochemical staining is an important method for a renal biopsy, which includes PAS staining, periodic acid silver methenamine (PASM) staining, and Masson staining. In this study, we aim to develop and modify the method of PASM and Masson double staining (PASM-Masson) in a renal biopsy. One hundred and fifty-two cases of renal biopsy were detected by PASM-Masson staining in our hospital. The results showed that the background of the slices was clean, and the renal structures were more visible under the microscope. The glomerular basement membrane, renal tubular basement membrane, and capillary basement membrane were stained black or brown; the mesangium and collagen fibers were stained blue black; the renal tubular epithelial cells and immune complexes were dyed red. These findings indicate that the glomerular immune complex deposition can be accurately identified and positioned by modified PASM-Masson staining, which might have important effects on the pathological diagnosis of renal diseases.
