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Isolement et culture des cellules endothéliales aortiques primaires des porcs miniatures
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Isolement et culture des cellules endothéliales aortiques primaires des porcs miniatures

Article DOI: 10.3791/59673-v • 06:38 min • August 14th, 2019
August 14th, 2019 •
1,2,3, 4, 5, 2, 6, 3, 3, 3, 1, 2, 1,2,3
1Department of Nephrology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 2Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, 3Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 4Department of Endocrinology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 5Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, 6People's Hospital of Longhua

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Une méthode enzymatique efficace pour l'isolement des cellules endothéliales aortiques primaires porcines (pAECs) des porcs miniatures est décrite. Les pAECs primaires isolés peuvent être employés pour étudier la réponse immunisée et de coagulation dans la xénotransplantation.

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Biologie Numéro 150 Cellules CD31 culture cellulaire porc miniature cellules endothéliales aortiques porcines isolement cellulaire primaire xénotransplantation
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