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Journal / Bioengineering / Analisi standardizzata e scalabile per studiare…
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Bioengineering

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JoVE Journal Bioengineering
Analisi standardizzata e scalabile per studiare la germogliazione angiogenica 3D perfusa di cellule endoteliali derivate da iPSC in Vitro
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Analisi standardizzata e scalabile per studiare la germogliazione angiogenica 3D perfusa di cellule endoteliali derivate da iPSC in Vitro

Article DOI: 10.3791/59678-v • 10:47 min • November 6th, 2019
November 6th, 2019 •
1,2, 1, 3, 3, 4, 5, 2, 1
1Department of Internal Medicine (Nephrology) and the Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, 2Division of Systems Biomedicine and Pharmacology, Department of Analytical BioSciences and Metabolomics, Leiden University, 3Ncardia, 4Department of Anatomy and Embryology, Leiden University Medical Center, 5Mimetas

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Questo metodo descrive la coltura delle cellule endoteliali derivate da iPSC come 40 micronavi 3D perfuse in una piattaforma microfluidica standardizzata. Questa piattaforma consente lo studio della germogliazione angiogenica basata su gradienti in 3D, tra cui l'anastomosis e la stabilizzazione dei germogli angiogenici in modo scalabile e ad alta produttività.

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Bioingegneria Problema 153 coltura di cellule 3D microfluidica in vitro cellule staminali pluripotenti indotte dall'uomo cellule endoteliali angiogenesi gradienti screening ad alto contenuto di produttività sviluppo di analisi
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