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JoVE Journal Neuroscience
Preparación de criosecciones retinianas de ratón para inmunohistoquímica
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Preparación de criosecciones retinianas de ratón para inmunohistoquímica

Article DOI: 10.3791/59683-v • 05:25 min • July 1st, 2019
July 1st, 2019 •
1, 2, 2, 1
1Department of Biomedical Sciences, University of Pennsylvania School of Veterinary Medicine, 2Division Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine, University of Pennsylvania School of Veterinary Medicine

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Este informe describe métodos completos para preparar secciones de retina de ratón congeladas para inmunohistoquímica (IHC). Los métodos descritos incluyen disección de la copa ocular posterior, fijación de paraformaldehído, incrustación en la temperatura de corte óptima (OCT) orientación de medios y tejidos, seccionamiento e inmunomancha.

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Neurociencia Número 149 Retina Cryo-section Medios de temperatura de corte óptimas OCT Inmunohistoquímica Enucleación de ojos de ratón
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