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Biochemistry

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JoVE Journal Biochemistry
Toepassing van Biolayer interferometrie (BLI) voor het bestuderen van eiwit-eiwit interacties in transcriptie
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Journal (Biochemistry)
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Separation of Polymerized Nucleic Acid Constructs by PAGE
Journal (Biochemistry)
Separation of Polymerized Nucleic Acid Constructs by PAGE
 
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Toepassing van Biolayer interferometrie (BLI) voor het bestuderen van eiwit-eiwit interacties in transcriptie

Article DOI: 10.3791/59687-v • 07:18 min • July 26th, 2019
July 26th, 2019 •
1,2, 3, 1,2
1Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers University, 2Graduate Program in Physiology and Integrative Biology, School of Graduate Studies, Rutgers University, 3Department of Plant Biology, School of Environmental and Biological, Rutgers University

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Interacties van transcriptiefactoren (TFs) met de RNA-polymerase worden meestal bestudeerd met behulp van keuze assays. We passen een Biolayer interferometrie (BLI) technologie toe om de interactie van GrgA met de chlamydiale RNA-polymerase te karakteriseren. Vergeleken met keuze assays, bli detecteert real-time associatie en dissociatie, biedt een hogere gevoeligheid, en is zeer kwantitatief.

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Deze maand in JoVE Biolayer interferometrie chlamydia CT504 TC0791 GrgA eiwit-eiwit interactie transcriptiefactoren transcriptie regulatie
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