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Neuroscience

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JoVE Journal Neuroscience
Forberedelse af nyfødte rotte hjernevæv til Ultrastrukturel morfometrisk analyse af Synaptic Vesicle distribution på nerve terminaler
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Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay
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Forberedelse af nyfødte rotte hjernevæv til Ultrastrukturel morfometrisk analyse af Synaptic Vesicle distribution på nerve terminaler

Article DOI: 10.3791/59694-v • 10:09 min • June 7th, 2019
June 7th, 2019 •
1,2, 1
1Department of Anesthesiology, University of Virginia Health System, 2Department of Anesthesiology, Universita' degli Studi di Padova

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Vi beskriver procedurer for behandling af nyfødte rotte hjernevæv for at opnå højopløsnings elektron mikrografer til morfometrisk analyse af synaptisk vesikel fordeling ved nerve terminaler. Mikrograferne opnået med disse metoder kan også bruges til at studere morfologien af en række andre cellulære komponenter og deres dimensionale strukturelle relationer.

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Neurovidenskab transmission elektronmikroskopi hjerne fiksation vaskulær perfusion heavy metal farvning harpiks indlejring sekventiel dehydreringen kontrast osmium uranylnitrat acetat bly
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