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JoVE Journal Neuroscience
Kvantifisere Subcellulære Ubiquitin-proteasomet aktivitet i gnager hjernen
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Kvantifisere Subcellulære Ubiquitin-proteasomet aktivitet i gnager hjernen

Article DOI: 10.3791/59695-v • 09:25 min • May 21st, 2019
May 21st, 2019 •
1, 2, 1,2
1Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, 2School of Neuroscience, Virginia Polytechnic Institute and State University

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Denne protokollen er utformet for å effektivt kvantifisere ubiquitin-proteasomet system (UPS) aktivitet i ulike cellulære rom i den gnager hjernen. Brukernes er kjøpedyktig eksamen UPS fungerer inne atomer, cytoplasmatiske og Synaptic brøk inne det likt dyr, slankende beløpet av tid og antallet av dyrene behøvde å utføre disse innviklet analyser.

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Nevrovitenskap ubiquitin proteasomet Nucleus synapse cytoplasma minne hippocampus amygdala
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