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Kwantificerende Subcellulaire Ubiquitin-proteasome activiteit in het knaagdier hersenen
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Kwantificerende Subcellulaire Ubiquitin-proteasome activiteit in het knaagdier hersenen

Article DOI: 10.3791/59695-v • 09:25 min • May 21st, 2019
May 21st, 2019 •
1, 2, 1,2
1Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, 2School of Neuroscience, Virginia Polytechnic Institute and State University

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Dit protocol is ontworpen om de activiteit van ubiquitin-proteasome System (UPS) in verschillende cellulaire compartimenten van de knaagdieren hersenen efficiënt te kwantificeren. Gebruikers zijn in staat om de werking van UPS in nucleaire, cytoplasmatische en synaptische fracties in hetzelfde dier te onderzoeken, waardoor de hoeveelheid tijd en het aantal dieren dat nodig is om deze complexe analyses uit te voeren, wordt verminderd.

Tags

Neuroscience probleem 147 ubiquitin proteasome Nucleus SYNAPS cytoplasma geheugen Hippocampus amygdala
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