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JoVE Journal Neuroscience
Kvantifiera subcellulär ubiquitin-Proteasome-aktivitet i gnagare hjärnan
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Kvantifiera subcellulär ubiquitin-Proteasome-aktivitet i gnagare hjärnan

Article DOI: 10.3791/59695-v • 09:25 min • May 21st, 2019
May 21st, 2019 •
1, 2, 1,2
1Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, 2School of Neuroscience, Virginia Polytechnic Institute and State University

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Detta protokoll är utformat för att effektivt kvantifiera ubiquitin-Proteasome system (UPS) aktivitet i olika cellulära fack i gnagare hjärnan. Användare kan undersöka UPS fungerar i nukleära, cytoplasmiska och synaptiska fraktioner i samma djur, minska mängden tid och antal djur som behövs för att utföra dessa komplexa analyser.

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Neurovetenskap ubiquitin Proteasome Nucleus Synapse cytoplasma minne Hippocampus amygdala
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