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Fysiologiske pasient avledet 3D Spheroids for anti-neoplastic Drug screening å målrette Cancer stamceller

Article DOI: 10.3791/59696-v • 10:03 min • July 5th, 2019
July 5th, 2019 •
*1, *1, *1, *2, *1, *2, *2, 1,2,3,4
1Department of Biomedical Engineering, University of Michigan, 2Department of Materials Science and Engineering, University of Michigan, 3Rogel Cancer Center, School of Medicine, University of Michigan, 4Macromolecular Science and Engineering, University of Michigan
* These authors contributed equally

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Denne protokollen beskriver generering av pasient-avledet spheroids, og nedstrøms analyse inkludert kvantifisering av spredning, cytotoksisitet testing, flyt flowcytometri, immunofluorescence farging og konfokalmikroskopi bildebehandling, for å vurdere narkotika potensielle neoplastic legemidler. Denne protokollen støtter presisjons medisin med identifisering av spesifikke medikamenter for hver pasient og stadium av sykdom.

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Kreftforskning pasient avledet kreft stamceller narkotika screening høy gjennomstrømning hengende dråpe spheroids presisjon medisin
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