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Journal / Immunology and Infection / Kvantificering af Monoyte Chemotaktisk aktivitet…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Kvantificering af Monoyte Chemotaktisk aktivitet in vivo og karakterisering af blod monocytter afledt makrofager
Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
Journal (Immunology and Infection)
Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
Journal (Immunology and Infection)
Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
 
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Kvantificering af Monoyte Chemotaktisk aktivitet in vivo og karakterisering af blod monocytter afledt makrofager

Article DOI: 10.3791/59706-v • 09:57 min • August 12th, 2019
August 12th, 2019 •
1, 1, 1,2
1Department of Internal Medicine, Section on Molecular Medicine, Wake Forest School of Medicine, 2Department of Biochemistry, Wake Forest School of Medicine

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Her præsenterer vi en protokol til at kvantificere den chemotaktiske aktivitet af blod monocytter i musemodeller, til at vurdere virkningerne af ernæringsmæssige, farmakologiske og genetiske interventioner på blod monocyt og til at karakterisere de blod monocytter afledte makrofager i mus modeller ved hjælp af monocyte-chemoattraktant protein-1 (MCP-1)-lastet kælder membran-afledte gel stik.

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Immunologi og infektion udstedelse 150 Matrigel MCP-1 Monocyte Macrophage enkelt celle Western blot musemodeller åreforkalkning
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